Beta-mammal toxin Css4 precursor - Centruroides suffusus (Durango bark scorpion)

Functionⁱ

Beta toxins bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on mammals.

3 Publications

Caution

Authors of PubMed:21329715 cite the nucleotide sequence HQ262493 as coding for Css8 (AC P0DL83), but the sequence corresponds to Css4. For this reason, the corresponding cross-reference is indicated in this entry.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Siteⁱ	43	May interact with the receptor site1 Publication	1
Siteⁱ	46	May interact with the receptor site1 Publication	1
Siteⁱ	47	May interact with the receptor site1 Publication	1
Siteⁱ	51	May interact with the receptor site1 Publication	1

GO - Molecular functionⁱ

sodium channel inhibitor activity Source: InterPro
toxin activity Source: UniProtKB-KW

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

defense response Source: InterPro
modulation by symbiont of host nervous system process Source: InterPro

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Molecular function

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBⁱ	8.B.1.2.2, the long (4c-c) scorpion toxin (l-st) superfamily

TCDBⁱ

8.B.1.2.2, the long (4c-c) scorpion toxin (l-st) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Beta-mammal toxin Css4 Alternative name(s): Css IV2 Publications Short name: CssIVCurated
Organismⁱ	Centruroides suffusus (Durango bark scorpion)
Taxonomic identifierⁱ	6880 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Centruroides

Keywords - Cellular componentⁱ

Secreted

Pathology & Biotechⁱ

Toxic doseⁱ

LD₅₀ is 0.12 µg/kg in mouse by intracerebroventricular injection and LD₅₀ is 115 µg/kg in mouse by subcutaneous injection.1 Publication

LD(100) is 3 µg/kg by intracranial injection into mice.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	26	N → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	27	S → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	28	Y → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	32	K → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	34	E → A: Induces a marked shift in the voltage dependence of activation of Drosophila DmNav1 (para) channels and a contraction paralysis in blowfly larvae. However, it retains its high affinity for rat brain neuronal membranes and is highly active on the SCN2A (Nav1.2) channel. 2 Publications	1
Mutagenesisⁱ	34	E → Q or R: Has little effect on the binding affinity to rat sodium channels. 2 Publications	1
Mutagenesisⁱ	36	F → A: 11.2-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	36	F → W: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	37	K → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	38	L → A: 157-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	38	L → I: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	40	D → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	41	N → A: 649-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	42	D → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	43	Y → A: 886-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	43	Y → W: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	45	L → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	46	R → A: 30.8-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	46	R → I: 39-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	46	R → Q: 14-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	47	E → A: 648-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	47	E → L: 45-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	47	E → Q: 394-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	47	E → R: 962-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	49	R → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	50	Q → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	51	Q → A: 10.8-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	51	Q → W: 35.8-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	54	K → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	59	Y → A: 1451-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	61	Y → A: 1533-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	63	F → A: 831-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	68	T → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	71	Y → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	72	E → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	73	Q → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	75	V → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	76	V → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	77	W → A: 87-fold decrease in binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	80	P → G: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	81	N → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	83	T → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1
Mutagenesisⁱ	85	N → A: Has little effect on the binding affinity to rat sodium channels. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 19	1 PublicationAdd BLAST		19
ChainⁱPRO_0000066778	20 – 85	Beta-mammal toxin Css41 PublicationAdd BLAST		66

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	31 ↔ 84	PROSITE-ProRule annotation
Disulfide bondⁱ	35 ↔ 60	PROSITE-ProRule annotation
Disulfide bondⁱ	44 ↔ 65	PROSITE-ProRule annotation
Disulfide bondⁱ	48 ↔ 67	PROSITE-ProRule annotation
Modified residueⁱ	85	Asparagine amideBy similarity	1

Keywords - PTMⁱ

Amidation, Disulfide bond

Expressionⁱ

Tissue specificityⁱ

Expressed by the venom gland.1 Publication

Structureⁱ

3D structure databases

AlphaFoldDBⁱ	P60266
SMRⁱ	P60266
ModBaseⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	20 – 85	LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST		66

Domainⁱ

Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).Curated

Sequence similaritiesⁱ

Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily.Curated

Keywords - Domainⁱ

Signal

Family and domain databases

Gene3Dⁱ	3.30.30.10, 1 hit
InterProⁱ	View protein in InterPro IPR044062, LCN-type_CS_alpha_beta_dom IPR003614, Scorpion_toxin-like IPR036574, Scorpion_toxin-like_sf IPR018218, Scorpion_toxinL
PRINTSⁱ	PR00285, SCORPNTOXIN
SMARTⁱ	View protein in SMART SM00505, Knot1, 1 hit
SUPFAMⁱ	SSF57095, SSF57095, 1 hit
PROSITEⁱ	View protein in PROSITE PS51863, LCN_CSAB, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

P60266-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MNSLLMITAC LALVGTVWAK EGYLVNSYTG CKFECFKLGD NDYCLRECRQ 
        60         70         80 
QYGKGSGGYC YAFGCWCTHL YEQAVVWPLP NKTCNGK

Length:87

Mass (Da):9,785

Last modified:November 22, 2017 - v2

Checksum:ⁱ3AD30B6651C0C238

GO

Mass spectrometryⁱ

Molecular mass is 7601.6 Da. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	HQ262493 mRNA Translation: ADY17425.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

HQ262493 mRNA Translation: ADY17425.1

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P60266	Toxin Cll2b		CENLI		87	UniRef90_P60266
Beta-toxin Cii1		CENII		66
Beta-toxin Cn4		CENNO		87
Beta-toxin Ct1a		CENTE		87
Toxin Cll4		CENLI		87
+2

Protein	Similar proteins		Species	Score	Length	Source
P60266	Beta-toxin Ct1a		CENTE		87	UniRef50_P60266
Beta-toxin Cn4		CENNO		87
Toxin Cll4		CENLI		87
Beta-toxin Cii1		CENII		66
Toxin CsEv1	)	CENSC		87
+52

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	HQ262493 mRNA Translation: ADY17425.1

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

HQ262493 mRNA Translation: ADY17425.1

3D structure databases

AlphaFoldDBⁱ	P60266
SMRⁱ	P60266
ModBaseⁱ	Search...

Protein family/group databases

TCDBⁱ	8.B.1.2.2, the long (4c-c) scorpion toxin (l-st) superfamily

TCDBⁱ

8.B.1.2.2, the long (4c-c) scorpion toxin (l-st) superfamily

Family and domain databases

Gene3Dⁱ	3.30.30.10, 1 hit
InterProⁱ	View protein in InterPro IPR044062, LCN-type_CS_alpha_beta_dom IPR003614, Scorpion_toxin-like IPR036574, Scorpion_toxin-like_sf IPR018218, Scorpion_toxinL
PRINTSⁱ	PR00285, SCORPNTOXIN
SMARTⁱ	View protein in SMART SM00505, Knot1, 1 hit
SUPFAMⁱ	SSF57095, SSF57095, 1 hit
PROSITEⁱ	View protein in PROSITE PS51863, LCN_CSAB, 1 hit
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SCX4_CENSU
Accessionⁱ	P60266Primary (citable) accession number: P60266 Secondary accession number(s): F1CGT5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
	Last sequence update:	November 22, 2017
	Last modified:	May 25, 2022
	This is version 67 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Animal Toxin Annotation Program

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UniParc

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Supporting data

UniProtKB - P60266 (SCX4_CENSU)

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Beta-mammal toxin Css4

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted

Extracellular region or secreted

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Family and domain databases

<p>This subsection of the 'Sequence' section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein family/group databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Mass spectrometryⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ